全文获取类型
收费全文 | 3187篇 |
免费 | 278篇 |
国内免费 | 169篇 |
专业分类
林业 | 418篇 |
农学 | 170篇 |
基础科学 | 16篇 |
132篇 | |
综合类 | 1321篇 |
农作物 | 96篇 |
水产渔业 | 793篇 |
畜牧兽医 | 346篇 |
园艺 | 215篇 |
植物保护 | 127篇 |
出版年
2024年 | 8篇 |
2023年 | 23篇 |
2022年 | 59篇 |
2021年 | 71篇 |
2020年 | 73篇 |
2019年 | 77篇 |
2018年 | 45篇 |
2017年 | 104篇 |
2016年 | 147篇 |
2015年 | 114篇 |
2014年 | 124篇 |
2013年 | 163篇 |
2012年 | 264篇 |
2011年 | 242篇 |
2010年 | 221篇 |
2009年 | 187篇 |
2008年 | 187篇 |
2007年 | 237篇 |
2006年 | 176篇 |
2005年 | 183篇 |
2004年 | 137篇 |
2003年 | 121篇 |
2002年 | 88篇 |
2001年 | 87篇 |
2000年 | 67篇 |
1999年 | 69篇 |
1998年 | 56篇 |
1997年 | 51篇 |
1996年 | 59篇 |
1995年 | 40篇 |
1994年 | 34篇 |
1993年 | 27篇 |
1992年 | 32篇 |
1991年 | 13篇 |
1990年 | 21篇 |
1989年 | 10篇 |
1988年 | 4篇 |
1987年 | 8篇 |
1986年 | 4篇 |
1981年 | 1篇 |
排序方式: 共有3634条查询结果,搜索用时 15 毫秒
101.
Guojian Jiang 《Aquaculture Research》2010,41(9):1322-1330
The defence ability of Fenneropenaeus chinensis and Marsupenaeus japonicus against the intrusion of the white spot syndrome virus (WSSV) was compared after injecting WSSV intramuscularly by recording cumulative mortality, diagnosing the virus and examining variations in immunological parameters including the total haemocyte counts (THCs), phagocytic percentage (PP), plasma protein concentration (PPC), phenoloxidase (PO) and nitric oxide synthase (NOS) activity. The results showed that the variations in the immunological parameters of F. chinensis and M. japonicus showed similar trends. The THCs of the two species decreased significantly postchallenge of WSSV. The virus was detected at 78 h in M. japonicus and at 42 h in F. chinensis after infection, which was in correlation with the accumulative mortality, and the variations in PO, PP, NOS and PPC in the two species. All shrimps of F. chinensis in the mortality experiment died within 66 h, much more quickly than M. japonicus, whose cumulative mortality reached 100% after 198 h. In conclusion, the immune system of M. japonicus has a stronger resistant ability to antagonize and endure the invasion of WSSV than that of F. chinensis. 相似文献
102.
The VP 28 gene encoding a structural envelope protein of the white spot syndrome virus (WSSV) was cloned into a pET32a(+) expression vector for the production of the recombinant VP28 protein. A purified recombinant protein of 39.9 kDa size was used for polyclonal antibody production in rabbit. Specific immunoreactivity of the rabbit anti rVP28 antiserum to the viral antigen was confirmed by a Western blot. The specificity of this polyclonal anti‐rVP28 antiserum to detect the presence of the virus in WSSV‐infected Penaeus monodon was verified using a immunodot blot assay. Immunodot blot showed a positive reaction in infected shrimp tissues with prominent colour development using 3,3′,5,5′‐tetramethylbenzidine (TMB) as a chromogenic substrate when compared with 3–3′ diaminobenzidine tetrahydrochloride (DAB). Highest signal intensities of the immunodots were observed in infected shrimp pleopod extracts and haemolymph. On comparison with polymerase chain reaction (PCR), immunodot blot could detect 76% of PCR‐positive WSSV‐infected shrimp samples. Immunodot blot was found to be equivalent to first‐step PCR sensitivity to detect WSSV particles estimated to contain 1.0 × 105 viral DNA copies. 相似文献
103.
Research on the little loligo squid (Uroteuthis chinensis) protein hydrolyzed by papain and Flavourzyme and characterization of hydrolysates was carried out in this article. The optimum hydrolysis conditions of papain were obtained by orthogonal experiments, and the influence factors on the degree of hydrolysis such as enzyme/substrate ratio, hydrolysis time, pH, and temperature were investigated. The effect of Flavourzyme on the degree of hydrolysis was also studied. The characteristics of freeze-dried hydrolysates such as yield, moisture, nitrogen recovery, molecular mass distribution, and amino acid composition were determined. The results showed that papain and Flavourzyme could hydrolyze the little loligo squid protein efficiently, and the degree of hydrolysis was up to 25.82% under the optimum hydrolysis conditions. The average molecular mass of hydrolysates obtained with papain and Flavourzyme was 1,246 Da, and the low molecular mass peptides were the main fraction of hydrolysates. The yield, moisture, and nitrogen recovery of freeze-dried hydrolysates was 19.8, 4.4, and 74.2%, respectively. Compared with suggested patterns of amino acid requirements by the Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO), the hydrolysates of the little loligo squid had high nutritional value, and was a potential nutritious supplement used in various food products. 相似文献
104.
Pattira Pongtippatee Roengsak Luppanakane Pinij Thaweethamsewee Pornpimol Kirirat Wattana Weerachatyanukul & Boonsirm Withyachumnarnkul 《Aquaculture Research》2010,41(2):227-232
The aim of this study was to determine whether magnesium (Mg2+ ) in seawater is required for egg activation of the black tiger shrimp Penaeus monodon and whether manipulation of Mg2+ levels can be used to delay the process and thereby synchronize egg activation. Female P. monodon broodstock were allowed to spawn in artificial seawater containing Mg2+ at varying levels with respect to the normal (100%) level: 100%, 50%, 20% and 0%. Egg activation occurred normally at 100% Mg2+ , incompletely at 50% and 20% Mg2+ levels and did not occur at all with 0% Mg2+ . The fertilization rate with 100% Mg2+ was observed to be 83%, but fertilization failed to take place in all the other groups. The fertilization rate was restored from 0% to 76% following the 20% Mg2+ level treatment when Mg2+ levels returned to normal (100%) as soon as spawning was completed. This study suggests that the level of Mg2+ in seawater plays a vital role in P. monodon egg activation, and that commencement of this process could be delayed by manipulation of the Mg2+ level during and immediately after spawning. 相似文献
105.
对五味子实生苗地下横走茎的形态发生、发育过程进行了研究。结果表明:1 a生五味子子叶腋芽可发育为地下横走茎,但其发育较缓慢,在8月下旬至9月初才发育成地下横走茎,第1片真叶腋芽和第2片真叶腋芽当年均可以发育成地下横走茎;2 a生和多年生五味子植株基部的越冬芽都会发育成地下横走茎;无论是子叶腋芽还是越冬芽在转变初期与一般芽形态相似,但经过伸长生长后,其茎尖开始弯曲以适应地下生长。地下横走茎的发生部位较集中,常呈轮生状。较高节位芽的超显微结构与子叶叶芽存在较大差异,说明五味子的芽能否形成地下横走茎在其形成的早期可能就已经决定。 相似文献
106.
斑节对虾白斑综合症病毒部分基因组文库及核酸探针检测法 总被引:12,自引:1,他引:11
通过分离纯化白斑综合症病毒(WSSV)粒子,抽提病毒DNA。用限制性内切酶EcoRⅠ或SalⅠ酶切后,克隆入质粒pBluescriptⅡKS中,从而建立了WSSV部分基因组文库。估计WSSV基因组DNA在165kb以上。将WSSV EcoRⅠ克隆片段标记制备为探针。进行Southern杂交、打点杂交和原位杂交,其结果证明了克隆片段对WSSV特异,并为检测WSSV提供了方法。通过对部分基因组文库序列 相似文献
107.
108.
109.
110.
不同密度的羊草种群结构及其光照分布的研究 总被引:8,自引:1,他引:7
本文报道了耕翻后自然更新的羊草种群不同密度条件下垂直结构及其光照分布特征。结果表明,地上生物量和叶面积指数均以种群中部最大,向上或向下皆逐渐减小。在三种密度中,生物量,叶面积指数,株高,茎高和叶片含水量均随着密度的增大而升高。比叶重和单株绿叶数度增加而降低。 相似文献